Staphylococcus aureus SarA is a regulatory protein responsive to redox and pH that can support bacteriophage lambda integrase-mediated excision/recombinationmmi_6942
نویسندگان
چکیده
David F. Fujimoto,1† Robin H. Higginbotham,2 Kristen M. Sterba,4 Soheila J. Maleki,2 Anca M. Segall,1 Mark S. Smeltzer3 and Barry K. Hurlburt2,4* Department of Biology, San Diego State University, San Diego, CA 92182, USA. United States Department of Agriculture, Agricultural Research Service, Southern Regional Research Center, New Orleans, LA 70124, USA. Departments of Microbiology and Immunology and Biochemistry and Molecular Biology, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA.
منابع مشابه
Staphylococcus aureus SarA is a regulatory protein responsive to redox and pH that can support bacteriophage lambda integrase-mediated excision/recombination.
Staphylococcus aureus produces a wide array of virulence factors and causes a correspondingly diverse array of infections. Production of these virulence factors is under the control of a complex network of global regulatory elements, one of which is sarA. sarA encodes a DNA binding protein that is considered to function as a transcription factor capable of acting as either a repressor or an act...
متن کاملInteractions between integrase and excisionase in the phage lambda excisive nucleoprotein complex.
Bacteriophage lambda site-specific recombination comprises two overall reactions, integration into and excision from the host chromosome. Lambda integrase (Int) carries out both reactions. During excision, excisionase (Xis) helps Int to bind DNA and introduces a bend in the DNA that facilitates formation of the proper excisive nucleoprotein complex. The carboxyl-terminal alpha-helix of Xis is t...
متن کاملRole of staphylococcal phage and SaPI integrase in intra- and interspecies SaPI transfer.
SaPIbov2 is a member of the SaPI family of staphylococcal pathogenicity islands and is very closely related to SaPIbov1. Typically, certain temperate phages can induce excision and replication of one or more of these islands and can package them into special small phage-like particles commensurate with their genome sizes (referred to as the excision-replication-packaging [ERP] cycle). We have s...
متن کاملMolecular Cloning, Expression and Peroxidase Conjugation of Staphylococcus aureus Protein A
Background: Staphylococcal protein A (SPA) is a cell wall component of Staphylococcus aureus that binds to different IgG subclasses of human and several animal species. This bacterial protein can be used as an antibody detector in various immunological assays or as an isolation reagent for the purification of antibody molecules via immuno-chromatography procedures.Objectives: Molecular cl...
متن کاملطراحی و ساخت نانو زیست ذرات فاژی نوترکیب به عنوان کاندیدای حامل واکسن ژنی- خوراکی
Background and Objective : Bacteriophage vectors recently have been considered as a gene transfer and vaccine delivery vehicles chiefly due to their low cost, safety, and physical stability. Since, little is known about phage mediated gene transfer in mammalian hosts, A group of invitro experiments were performed to ascertain gene transfercapability of these vehicles . Materials and Methods...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2009